The “Enzymes, Proteolysis & Metabolism at the crossroads of Biochemistry” Congress is the first joint meeting of three Thematic Groups of the SFBBM. It will be held at Université Paris Cité (Buffon Amphitheater) from December 3 to 5, 2025, and will bring together French and international communities working on enzymes, proteolysis, and metabolism. At the crossroads of fundamental biochemistry and its biomedical applications, this event will provide a unique platform to present recent advances, foster interdisciplinary exchanges, stimulate new collaborations, and highlight the central role of these topics in understanding biological mechanisms and human pathologies.